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. 2001 Jan 23;98(3):1261–1266. doi: 10.1073/pnas.031573798

Figure 3.

Figure 3

Depolymerization of actin filaments causes internalization of AMPARs. (A) Internalization of AMPAR is blocked by prior incubation with jasplakinolide (jas). Cultures were first treated with 2 μM jas for 2 h before they were labeled with GluR1 antibody, and treated with control or glutamate-containing solutions. Glutamate-induced internalization is blocked by jas, whereas jas by itself has no effect (A1, n = 12, 15, 16, and 15 cells for control, jas, jas + Glu, and Glu, respectively, from four experiments). This effect may be due, at least in part, to an inhibition of endocytosis by jas as the uptake of Tf is also reduced (A2, n = 45 and 42 cells for control and jas, respectively, from five experiments). (B) Latrunculin A (lat A) mimics the effect of glutamate on AMPAR internalization. Cultures were incubated with lat A for 1 h at the concentration indicated, and the distribution of AMPAR was evaluated as above. Lat A causes internalization of AMPAR in a dose-dependent manner (n = 19, 19, 21, 15, 14, and 16 cells for control, 5 μM, 10 μM, 20 μM, 50 μM, and glutamate, respectively, from four experiments).