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Insulin increases the rate of endocytosis. FM1–43 and Tf labeling were used to measure the effects of insulin (5 μM) on the rate of endocytosis. An increased uptake of FM1–43 (A: n = 12 cells from 11 experiments) and Tf (B: n = 34 and 25 cells for control and insulin from 5 experiments) is detected, which is accompanied by an enhanced internalization of AMPARs (C: n = 16 and 14 cells for control and insulin, from 3 experiments).
