Figure 8. Single-channel kinetics of TRPV1 channel activity induced by RTX and capsaicin in cell-attached patches from DRG neurones.

A, open- and closed-time distributions in the presence of RTX (0.1 nm) at −60 mV and +60 mV; open and closed time could be well-fitted with three exponential components. The time constants and the relative areas of distribution (in parenthesis) are shown. B, at −60 mV, in the presence of capsaicin (100 nm) the closed-time distribution is well-fitted with four exponential components and the open-time distribution is well-fitted with three exponential components. At +60 mV, the closed- and open-time distributions are well fitted with four exponential components. In the presence of RTX, the channel predominantly dwelled in the longer open states the majority of the time as compared to activation by capsaicin.