Histological analyses of perirenal fat pads of 21-day-old rat pups born to mothers fed either chow ad libitum during gestation and lactation (C, □), the cafeteria diet during gestation followed by chow ad libitum during lactation (CDG, ░) or the cafeteria diet during gestation and lactation (CDW, ▒). A, H&E stained 6 μm thick sections from each nutrition group. B, adipocyte area results. C, an estimation of adipocyte numbers. Results are means ±s.e.m., n = 5 litters; different letters indicate statistically significant differences (P < 0.05), by ANOVA followed by Tukey's post hoc analysis. Scale bars are 50 μm.