Figure 4. A maternal cafeteria diet affects the expression of genes involved in muscle growth and metabolism in the offspring.

Relative gene expression analyses using real-time PCR and SyBR green detection in the gastrocnemius muscles of 21-day-old rat pups born to mothers fed either chow ad libitum during gestation and lactation (C, □), the cafeteria diet during gestation followed by chow ad libitum during lactation (CDG, ░) or the cafeteria diet during gestation and lactation (CDW, ▒). Results are means ±s.e.m., n = 6; different letters indicate statistically significant differences (P < 0.05), *0.05 ≤P≤ 0.10 by ANOVA followed by Tukey's post hoc analysis.