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. 2001 Jan 23;98(3):1267–1272. doi: 10.1073/pnas.031461598

Figure 4.

Figure 4

Transduction and effect of TAT-CRAC on MA-10 Leydig cells. Fluorescent microscopy pictures of MA-10 cells treated for 30 min with either Oregon Green 488 (A) or 10 μM Oregon Green 488-labeled TAT-CRAC (B). MA-10 Leydig cells were treated with the indicated concentration of TAT-CRAC or TAT-mCRAC for 30 min. Cells were then exposed to 50 ng/ml hCG (C) or 20 μM 22R-hydroxycholesterol (D) for 2 h. At the end of the incubation, media were collected, and progesterone levels were determined by RIA. Results shown are means ±SD from four independent experiments (n = 12).