Abstract
The toxic effects of gas-phase singlet oxygen, 1O2, on the ciliated respiratory epithelium of hamster trachea have been demonstrated. Tracheal explants treated with 1O2 showed a dose-dependent decrease in cilia beating frequency and focal ciliostasis. A statistically significant decrease in ciliary activity occurred at 1O2 concentrations as low as 154 ppb after a 2-hr exposure. Cytological alterations in the mucociliary epithelium were observed in explants exposed to 235 ppb 1O2 or greater. When cytotoxic effects were related to the time of exposure to 1O2, maximum effects occurred after a 4-hr exposure. In vitro recovery studies indicate that ciliary activity returned to normal between 4 and 8 hr after exposure.
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Selected References
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- Adalis D., Gardner D. E., Miller F. J., Coffin D. L. Toxic effects of cadmium on ciliary activity using a tracheal ring model system. Environ Res. 1977 Feb;13(1):111–120. doi: 10.1016/0013-9351(77)90009-3. [DOI] [PubMed] [Google Scholar]
- Eisenberg W. C., Taylor K., Schiff L. J. Biological effects of singlet delta oxygen on respiratory tract epithelium. Experientia. 1984 May 15;40(5):514–515. doi: 10.1007/BF01952414. [DOI] [PubMed] [Google Scholar]
- Gabridge M. G., Meccoli R. A. Cytotoxicity and ciliostasis in tracheal explants exposed to cadmium salts. Environ Health Perspect. 1982 Apr;44:189–195. doi: 10.1289/ehp.8244189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grose E. C., Gardner D. E., Miller F. J. Response of ciliated epithelium to ozone and sulfuric acid. Environ Res. 1980 Aug;22(2):377–385. doi: 10.1016/0013-9351(80)90149-8. [DOI] [PubMed] [Google Scholar]
- Schiff L. J., Bryne M. M., Fenters J. D., Graham J. A., Gardner D. E. Cytotoxic effects of sulfuric acid mist, carbon particulates, and their mixtures on hamster tracheal epithelium. Environ Res. 1979 Aug;19(2):339–354. doi: 10.1016/0013-9351(79)90060-4. [DOI] [PubMed] [Google Scholar]
- Schiff L. J., Byrne M. M., Graham J. A. Fly ash-induced changes in hamster tracheal epithelium in vivo and in vitro. J Toxicol Environ Health. 1981 Sep;8(3):431–448. doi: 10.1080/15287398109530081. [DOI] [PubMed] [Google Scholar]
- Schiff L. J. Effect of nitrogen dioxide on influenza virus infection in hamster trachea organ culture. Proc Soc Exp Biol Med. 1977 Dec;156(3):546–549. doi: 10.3181/00379727-156-39977. [DOI] [PubMed] [Google Scholar]
- Schiff L. J., Eisenberg W. C., Taylor K. Evidence of DNA repair in organ cultures of hamster tracheal epithelium following exposure to gas phase singlet oxygen. Mutat Res. 1985 Jan-Feb;142(1-2):41–44. doi: 10.1016/s0165-7992(85)80010-5. [DOI] [PubMed] [Google Scholar]
- Schiff L. J. Studies on the mechanisms of influenza virus infection in hamster trachea organ culture. Correlation of reduced viral adsorption with age-related resistance to infection. Arch Gesamte Virusforsch. 1974;44(3):195–204. doi: 10.1007/BF01240607. [DOI] [PubMed] [Google Scholar]
- Weishaupt K. R., Gomer C. J., Dougherty T. J. Identification of singlet oxygen as the cytotoxic agent in photoinactivation of a murine tumor. Cancer Res. 1976 Jul;36(7 Pt 1):2326–2329. [PubMed] [Google Scholar]