5-HT, 5-HIAA, and 5-HT turnover (5-HIAA/5-HT ratio) determinations by
HPLC in cerebral cortex (Cor), hypothalamus (Hyp), hippocampus (Hipp),
amygdala (Amy), midbrain (Mid), and cerebellum (Cer) of
nNOS−/− mice as compared with WT. The levels of 5-HT,
5-HIAA (ng/mg tissue), and 5-HT turnover in WT mice are as follows
(±SEM): Cor = 0.349 ± 0.012, 0.134 ± 0.005,
0.386 ± 0.018 (n = 6); Hyp = 1.200
± 0.038, 0.683 ± 0.043, 0.569 ± 0.032
(n = 6); hipp = 0.614 ± 0.019,
0.351 ± 0.027, 0.571 ± 0.038 (n = 6);
Amy = 0.807 ± 0.056, 0.364 ± 0.023, 0.470 ±
0.060 (n = 6); Mid = 0.949 ± 0.023,
0.557 ± 0.035, 0.586 ± 0.031 (n = 5);
Cer = 0.093 ± 0.005, 0.100 ± 0.005, 1.094 ±
0.086 (n = 6), respectively. In
nNOS−/− mice, n = 6 for Hyp, Hipp,
Mid, and Cer; n = 5 for Cor and Amy. Data are
percent change in relation to WT mice ± SEM; *,
P < 0.05 by Student's t test.