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. 2001 Jan 16;98(3):1277–1281. doi: 10.1073/pnas.031487198

Figure 2.

Figure 2

Reduction in aggressive behavior and increase in 5-HT metabolism by the precursor 5-HTP. (A and B) Aggressive behavior as measured by the resident–intruder test 30 min after 0.9% saline solution, 50 mg/kg or 100 mg/kg 5-HTP i.p. injections in WT and nNOS−/− mice (n = 6 each group). (C) Locomotor activity in an open field 30 min after i.p. injections in different animals (saline and 5-HTP 100 mg/kg, n = 6 in each genotype; and 5-HTP 50 mg/kg, n = 5 in each genotype). *, P < 0.05; and †, P < 0.01 in relation to the saline group in the same genotype; and #, P < 0.05; and ##, P < 0.01 in relation to WT same treatment. Data are means ± SEM and were analyzed by using two-way ANOVA (genotype × treatment) with post hoc Tukey test. (D) HPLC determinations of 5-HT (S) and its metabolite 5-HIAA (M) in the cerebral cortex (Cor), hypothalamus (Hyp), hippocampus (Hipp), amygdala (Amy), midbrain (Mid), and cerebellum (Cer) of WT and nNOS−/− mice (n = 5 each genotype) 45 min after 100 mg/kg 5-HTP. Data are percent increase above saline groups in each genotype (n = 6 each; means ± SEM). The percent of turnover increase (5-HIAA/5-HT) ranged from 346% to 646% in these brain areas. All data in D are P < 0.01 in relation to saline controls (two-way ANOVA with post hoc Tukey test).