Figure 2.
Cloning Strategy for Sequencing and Genetic Organization of val Gene Cluster. (A) Cloning strategy for sequencing of the 45 kb region. B: BamHI; C: ClaI; E: EcoRI. (B) Genetic organization of the val gene cluster. Genes proposed to be involved in validamycin biosynthesis are indicated as black arrows. The previously sequenced 6.0 kb BamHI fragment was indicated by double-arrowed line.