Figure 2.
Effect of infection of rat VSMCs in culture by recombinant adenovirus. (A) Subconfluent (104) rat VSMCs were infected with ADV-β-gal or ADV-α s* at a moi of 200. Uninfected cells were used as controls. Initial infections were conducted in 6-well plates (for early measurements), as well as 10-cm plates (for later measurements). After infection, cells were counted in triplicate every day for 6 days. Data are presented as the mean ± SE (*, P < 0.05 ADV-αs*-treated cells vs. control and ADV-β-gal-treated cells; **, P < 0.05 ADV-β-gal-treated cells vs. unifected control and ADV-αs* cells; ANOVA). (B) Phase contrast pictures (×100) taken on day 6 are also shown.