Figure 9. Summary of cell volume measurements.

NHE-deficient and parent MDCK-F cells were exposed to 140 mm sodium propionate in the absence and presence of the NBC inhibitor S0859 (50 μm). Cells were incubated in CO₂/HCO₃⁻- (A) or Hepes-buffered Ringer solution (B). Exposure to sodium propionate causes cell swelling of NHE-deficient MDCK-F cells only when they are incubated in CO₂/HCO₃⁻-buffered Ringer solution. Cell swelling is inhibited by the NBC1 blocker S0859. Parent MDCK-F cells also swell in Hepes-buffered Ringer solution. (Data for MDCK-F cells in B are taken from Klein et al. (2000)). *Significantly different (P < 0.05) from the respective control period.