Table 3.
Displacement of parent MDCK-F cells (NHE+) and NHE-deficient MDCK-F cells (NHE−) in different time intervals
| 30 min | 60 min | 90 min | |
|---|---|---|---|
| MDCK-F, NHE+ (pH 7.4) | |||
| Hepes (n = 27) | 23.1 ± 1.8 | 58.0 ± 3.7 | 91.0 ± 5.8 |
| CO2/HCO3− (n = 28) | 27.1 ± 2.3 | 62.1 ± 4.5 | 96.0 ± 6.5 |
| MDCK-F, NHE+ (pH 7.1) | |||
| Hepes (n = 33) | 27.2 ± 1.8 | 58.9 ± 3.9 | 78.9 ± 5.6 |
| CO2/HCO3− (n = 27) | 24.8 ± 2.4 | 63.0 ± 4.1 | 96.9 ± 5.6 |
| MDCK-F, NHE+, Hoe642 (pH 7.4) | |||
| Hepes (n = 33) | 22.4 ± 2.2 | 49.8 ± 4.4 | 70.0 ± 5.7 |
| CO2/HCO3− (n = 34) | 20.5 ± 1.7 | 49.4 ± 5.0 | 69.0 ± 7.2 |
| MDCK-F, NHE− (pH 7.4) | |||
| Hepes (n = 31) | 15.3 ± 1.2 | 40.6 ± 5.3 | 59.4 ± 5.3 |
| CO2/HCO3− (n = 42) | 22.0 ± 1.3 * | 47.9 ± 3.0 | 67.2 ± 4.6 |
| MDCK-F, NHE− (pH 7.1) | |||
| Hepes (n = 35) | 14.3 ± 1.4 | 36.9 ± 3.6 | 53.6 ± 5.4 |
| CO2/HCO3− (n = 49) | 19.0 ± 1.3 * | 43.7 ± 2.7 | 60.4 ± 5.4 |
*
Statistically significant differences (P < 0.05) between migration in Hepes- or CO2/HCO3−-buffered medium. The distances (in μm) covered within the indicated time intervals are listed.