Table 3.

Voltage dependence of activation of mutants in the IIS2 and IIS3 segments

	V1,½ (mV)	k1 (mV)	A1	V2,½ (mV)	k2 (mV)	A2	n
E805R-Control	−21.6 ± 0.3	8.2 ± 0.3	—	—	—	—	15
E805R-NP-Css IV 50 nm	−16.1 ± 1.3	6.6 ± 0.9	0.79 ± 0.11	−49.4 ± 1.9	10.1 ± 1.7	0.21 ± 0.08	18
E805R-Pre-Css IV 50 nm	−15.7 ± 1.6	4.3 ± 1.3	0.37 ± 0.08	−46.1 ± 2.9	8.9 ± 1.3	0.62 ± 0.8	20
D814R-Control	−26.2 ± 0.3	9.2 ± 0.2	—	—	—	—	7
D814R-NP-Css IV 50 nm	−22.9 ± 2.2	6.9 ± 2.6	0.71 ± 0.10	−45.2 ± 1.9	13.9 ± 1.6	0.28 ± 0.01	5
D814R-Pre-Css IV 50 nm	−23.9 ± 2.1	7.9 ± 1.9	0.55 ± 0.07	−52.3 ± 1.9	11.1 ± 1.7	0.44 ± 0.01	12
E821R-Control	−29.8 ± 0.3	5.6 ± 0.3	—	—	—	—	7
E821R-NP-Css IV 50 nm	−26.6 ± 2.2	5.9 ± 3.2	0.67 ± 0.05	−51.4 ± 3.2	14.2 ± 2.8	0.32 ± 0.02	7
E821R-Pre-Css IV 50 nm	−24.4 ± 2.1	6.1 ± 1.4	0.46 ± 0.14	−55.1 ± 0.8	11.1 ± 0.7	0.53 ± 0.01	9
D827R-Control	−18.9 ± 0.3	9.2 ± 0.2	—	—	—	—	13
D827R-NP-Css IV 50 nm	−28.6 ± 0.6	11.9 ± 0.6	—	—	—	—	11
D827R-Pre-Css IV 50 nm	−30.3 ± 0.7	10.1 ± 0.7	—	—	—	—	11