Figure 3. Changes in the extracellular concentration of the ACh-lc affect spontaneous [Ca²⁺]_i spiking activity.

AChE blocked spiking activity (A), whereas edrophonium increased its frequency (B). Trace C is representative of the cell population in which [Ca²⁺]_i spiking activity was not affected by AChE but it was blocked by subsequent addition of α-BuTX. In A and C each trace interruption represents an interval of 15 min. Results are from three different cells.