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. 2005 Jul 28;568(Pt 1):47–59. doi: 10.1113/jphysiol.2005.091199

Figure 1. Immunoblotting analysis of brain homogenates (h) and purified mitochondrial fractions (m) with polyclonal antibodies against fragments of SUR1 and SUR2, and different epitopes of Kir 6.1 and Kir 6.2, revealed multiple immunoreactive bands.

Figure 1

Blocking peptides (+peptide) were used to discriminate nonspecific bands. Blocking-peptide-insensitive 13 and 30 kDa bands, seen in A and B with anti-SUR antibodies, were also observed in all immunoblots with anti-Kir 6.X antibodies (not shown).