Figure 7.

Effect of hVps24 and TSG101 ablation on the vesicular pH of EGF-containing endocytic vesicles. Ratiometric fluorescence video image analysis of internalized EGF-FITC (and transferrin-FITC; our unpublished data) containing vesicles was performed in live HeLa cells transfected with the indicated siRNA as described in Materials and Methods. The mean pH of vesicle population was calculated by the Origin software, measured in five independent experiments (±SEM). In each experiment, the luminal pH of 100–300 endocytic vesicles was determined.