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Effect of Rac regulators on membrane targeting of GFP-Rac. (A) Postnuclear lysates from NIH3T3 cells expressing the indicated constructs were separated into membrane (M) and cytosolic (C) fractions. Equal percentages of cytosolic and membrane material were resolved by SDS-PAGE and immunoblotted for GFP (Rac), and integrin β1 and RhoGDI as membrane and cytosol markers, respectively. (B) Percentage of GFP-Rac in the membrane fraction; values are means ± SD (n = 3).
