Table 1.
Strain list
| Strain | Genotype |
|---|---|
| NY603 | MATα leu2-3,112 ura3-52 pep4::URA3 GAL+ |
| NY1060 | MATa/α ura3-52/ura3-52; leu2-3,112/leu2-3,112 GAL+ |
| NY1562 | MATa/α ura3-52::[URA3 GAL1p-SEC15; pNB304]/ura3-52; leu2-3,112 leu2-3,112 GAL+ |
| NY2145 | MATasec2-Δ1::HIS3 leu2-3,112::[LEU2 SEC2;pNB977] his3-Δ200 ura3-52 GAL+ |
| NY2146 | MATasec2-Δ1::HIS3 leu2-3,112::[LEU2 SEC2-GFP;pNB985] his3-Δ200 ura3-52 GAL+ |
| NY2147 | MATasec2-Δ1::HIS3 leu2-3,112::[LEU2 sec2-59-GFP;pNB990] his3-Δ200 ura3-52 GAL+ |
| NY2152 | MATasec2-Δ1::HIS3 leu2-3,112::[LEU2 sec2-78-GFP;pNB991] his3-Δ200 ura3-52 GAL+ |
| NY2432 | MATα leu2-3,112::[LEU2 GAL1p-GST-SEC2; pNB1153] ura3-52 pep4::URA3 GAL+ |
| NY2433 | MATα leu2-3,112::[LEU2 GAL1p-GST; pNB1154] ura3-52 pep4::URA3 GAL+ |
| NY2434 | MATα leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.1-160); pNB1157] ura3-52 pep4::URA3 GAL+ |
| NY2435 | MATα leu2-3,112::[LEU2 GAL1p-GST-sec2-59(aa.1-374); pNB1155] ura3-52 pep4::URA3 GAL+ |
| NY2436 | MATα leu2-3,112::[LEU2 GAL1p-GST-sec2-70(aa.1-508); pNB1156] ura3-52 pep4::URA3 GAL+ |
| NY2437 | MATα leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.161-759); pNB1159] ura3-52 pep4::URA3 GAL+ |
| NY2438 | MATα leu2-3,112::[LEU2 GAL1p-GST-sec2-78; pNB1160] ura3-52 pep4::URA3 GAL+ |
| NY2439 | MATα leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.1-450); pNB1161] ura3-52 pep4::URA3 GAL+ |
| NY2440 | MATaleu2-3,112 his3200 ura3-52 SEC3-GFP [URA] GAL+ |
| NY2441 | MATaleu2-3,112 his3Δ200 ura3-52 SEC5-GFP [URA] GAL+ |
| NY2443 | MATaleu2-3,112 his3Δ200 ura3-52 SEC8-GFP [URA] GAL+ |
| NY2446 | MATaleu2-3,112 his3Δ200 ura3-52 EXO70-GFP [URA] GAL+ |
| NY2520 | MATα leu2-3,112::[LEU2 EXO70-TAP] his3−Δ200::[HIS3 SEC5-3×HA kanMX6] ura3-52 GAL+ |
| NY2522 | MATα leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.1-258);pNB1236] ura3-52 pep4::URA3 GAL+ |
| NY2523 | MATα leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.161-258);pNB1237] ura3-52 pep4::URA3 GAL+ |
| NY2524 | MATα leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.161-374);pNB1238] ura3-52 pep4::URA3 GAL+ |
| NY2525 | MATa/α ura3-52::[URA3 GAL1p-SEC15; pNB304][/ura3-52; leu2-3,112::[LEU2 GAL1p-GST-SEC2; pNB1153]/leu2-3,112 GAL+ |
| NY2526 | MATa/α ura3-52::[URA3 GAL1p-SEC15]; pNB304]/ura3-52; leu2-3,112::[LEU2 GAL1p-GST-SEC10;pNB1239]/leu2-3,112 GAL+ |
| NY2527 | MATa/α ura3-52::[URA3 GAL1p-SEC15]; pNB304]/ura3-52; leu2-3,112::[LEU2 GAL1p-GST; pNB1154]/leu2-3,112 GAL+ |
| NY2528 | MATa/α ura3-52/ura3-52; leu2-3,112::[LEU2 GAL1p-GST-SEC2; pNB1153]/leu2-3,112 GAL+ |
| NY2529 | MATa/α ura3-52/ura3-52; leu2-3,112::[LEU2 GAL1p-GST-SEC10; pNB1239]/leu2-3,112 GAL+ |
| NY2530 | MATa/α ura3-52/ura3-52; leu2-3,112::[LEU2 GAL1p-GST; pNB1154]/leu2-3,112 GAL+ |
| NY2531 | MATaura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112 GAL+ |
| NY2532 | MATα ura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST-SEC2; pNB1153] GAL+ |
| NY2533 | MATα ura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST-SEC10;pNB1239] GAL+ |
| NY2534 | MATα ura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST; pNB1154] GAL+ |
| NY2535 | MATα ura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.1-160); pNB1157] GAL+ |
| NY2536 | MATα ura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST-sec2-59(aa.1-374); pNB1155] GAL+ |
| NY2537 | MATα ura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.1-450); pNB1161] GAL+ |
| NY2538 | MATα ura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST-sec2-70(aa.1-508); pNB1156] GAL+ |
| NY2539 | MATaura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.161-759); pNB1159] GAL+ |
| NY2540 | MATα ura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST-sec2-78; pNB1160] GAL+ |
| NY2541 | MATα ura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.1-258);pNB1236][ GAL+ |
| NY2542 | MATaura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.161-258);pNB1237] GAL+ |
| NY2543 | MATaura3-52::[URA3 GAL1p-SEC15; pNB304]; leu2-3,112::[LEU2 GAL1p-GST-sec2(aa.161-374);pNB1238] GAL+ |
| NY2544 | MATa/α ura3-52/ura3-52; leu2-3,112/leu2-3,112 his3/HIS3 GAL+ |
| NY2545 | MATα ura3-52 leu2-3,112 his3 Sec15-13×myc kanMX6 GAL+ |
| NY2546 | MATasec2-Δ1::HIS3 ura3-52 leu2-3,112::[LEU2 SEC2; pNB977] his3 Sec15-13×myc kanMX6 GAL+ |
| NY2547 | MATasec2-Δ1::HIS3 ura3-52 leu2-3,112::[LEU2 SEC2-GFP; pNB985] his3 Sec15-13×myc kanMX6 GAL+ |
| NY2548 | MATasec2-Δ1::HIS3 ura3-52 leu2-3,112::[LEU2 sec2-59-GFP;pNB990] his3 Sec15-13×myc kanMX6 GAL+ |
| NY2549 | MATasec2-Δ1::HIS3 ura3-52 leu2-3,112::[LEU2 sec2-78-GFP;pNB991] his3Sec15-13×myc kanMX6 GAL+ |
| NY2550 | MATa/α ura3-52::[URA3 GAL1p-SEC6;pNB1240][/ura3-52; leu2-3,112::[LEU2 GAL1p-GST-SEC2; pNB1153]/leu2-3,112 GAL+ |
| NY2551 | MATa/α ura3-52::[URA3 GAL1p-SEC6];pNB1240]/ura3-52; leu2-3,112::[LEU2 GAL1p-GST-SEC10;pNB1239]/leu2-3,112 GAL+ |
| NY2552 | MATa/α ura3-52::[URA3 GAL1p-SEC6];pNB1240]/ura3-52; leu2-3,112::[LEU2 GAL1p-GST; pNB1154]/leu2-3,112 GAL+ |
| NY2586 | MAT sec2-Δ1::HIS3 leu2-3,112::[LEU2 SEC2-GFP; pNB985] his3 ura3-52::[URA3 GAL1p-SEC15] GAL+ |
| SFNY1311 | MATaura3-52 leu2-3,112, his3-Δ200::[HIS GAL1p GST-GEA2] |
| SFNY1586 | MATaura3-1 leu2-3,112 trp1-1 ade2-1 can1-100 VPS1::[GAL1p GST-VPS1, HIS3] |
| NY2638 | MATα leu2-3,112 his3Δ200 ura3-52 SEC3-GFP [URA] sec2-59 GAL+ |
| NY2639 | MAT his3Δ200 ura3-52 SEC5-GFP [URA] sec2-59 GAL+ |
| NY2640 | MAT leu2-3,112 ura3-52 SEC8-GFP [URA] sec2-59 GAL+ |
| NY2641 | MATα leu2-3,112 his3Δ200 ura3-52 EXO70-GFP [URA] sec2-59 GAL+ |
| NY2642 | MAT sec2-Δ1::HIS3 ura3-52 leu2-3,112::[LEU2 SEC2-GFP; pNB985] his3 Sec15-13×myc kanMX6 elp1Δ kanMX6 GAL+ |
| NY2643 | MAT sec2-Δ1::HIS3 ura3-52 leu2-3,112::[LEU2 sec2-59-GFP;pNB990] his3 Sec15-13×myc kanMX6 elp1Δ kanMX6 GAL+ |
| NY2644 | MAT sec2-Δ1::HIS3 ura3-52 leu2-3,112::[LEU2 sec2-78-GFP;pNB991] his3 Sec15-13×myc kanMX6 elp1Δ kanMX6 GAL+ |
| NY2645 | MATasec2-Δ1::HIS3 leu2-3,112::[LEU2 sec2-59-GFP;pNB990] his3-Δ200 ura3-52 elp1Δ kanMX6 GAL+ |
| NY2646 | MATα sec2-Δ1::HIS3 ura3-52 leu2-3,112::[LEU2 SEC2-GFP; pNB985] his3 Sec15-13×myc kanMX6 sec5-24 GAL+ |