Figure 2.

The N-terminal region of RINT-1 interacts with ZW10. (A) Schematic representation of RINT-1 and its deletion constructs. The interaction of RINT-1 or its deletion constructs with ZW10 in yeast cells was evaluated by filter assays for β-galactosidase. Blue color was detected within 1 h (+++), 2 h (++) and 16 h (+). Expression of RINT-1C in yeast cells was confirmed by immunoblotting, whereas this fragment was not expressed in 293T cells (unpublished data). (B) 293T cells were transfected with the plasmids encoding the indicated FLAG-tagged constructs. At 24 h after transfection, cell lysates were prepared, and FLAG-tagged proteins were immunoprecipitated with an anti-FLAG antibody. The precipitated proteins were analyzed by immunoblotting with antibodies against ZW10 and FLAG (lanes 6–10). Lanes 1–5 show the amounts of ZW10 and FLAG-tagged proteins in 4% of the lysate. H.C. and L.C. denote immunoglobulin heavy and light chains, respectively.