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Delay in VSVG-GFP transport from the ER in cells with reduced expression of RINT-1. HeLa cells were successively transfected with siRNA and the plasmid for VSVG-GFP. Transport of VSVG-GFP from the ER was monitored as described in Materials and Methods. Typical images at 0, 30, 60, and 120 min after the temperature shift to 32°C are shown. N indicates the position of the nucleus. Bar, 5 μm. The quantitative results are shown at the bottom.
