Figure 6.

Retrograde transport is not significantly impaired in cells with reduced expression of RINT-1. (A) HeLa cells were transfected without (mock) or with RINT-1 (1149). At 72 h after transfection, the cells were incubated with 10 μM BFA for the indicated times, fixed, and stained with an antibody against Man II. Bar, 5 μm. (B) At 54 h after transfection, the cells were further transfected with the plasmids for Sar1p mutants and incubated for 18 h. Redistribution of Man II to the ER occurred in almost all RINT-1–depleted and control cells irrespective of the expression levels of Sar1p mutants (unpublished data). Bar, 5 μm. (C) RINT-1 or β-COP expression was knocked down by RNAi. The expression level of β-COP was decreased by 86% (unpublished data). The cells were incubated with 10 μM BFA for 30 min, fixed, and double-stained with antibodies against Man II and ERGIC-53. Bar, 5 μm.