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. 2006 Jun;17(6):2780–2788. doi: 10.1091/mbc.E05-10-0973

Figure 7.

Figure 7.

Silencing of RINT-1 causes a decrease in the amount of ZW10 associated with syntaxin 18. (A) HeLa cells were transfected without (mock) or with RINT-1 (1149) or lamin A/C siRNA. At 72 h after transfection, lysates were prepared and immunoprecipitated with an anti-syntaxin 18 antibody. The precipitated proteins were detected by immunoblotting with the indicated antibodies (lanes 4–6). Input (4% of total) was also analyzed (lanes 1–3). (B) Cell lysates were prepared as described in A, and immunoprecipitated with an anti-p31 (lanes 3 and 4), anti-BNIP1 (lanes 5 and 6), or control IgG (lanes 7 and 8). Input (2.8% of total) was also analyzed (lanes 1 and 2). (C) HeLa cells were transfected without (mock) or with lamin A/C siRNA or ZW10 (102). At 72 h after transfection, lysates were prepared and immunoprecipitated with an anti-syntaxin 18 antibody. The precipitated proteins were analyzed by immunoblotting with the indicated antibodies (lanes 4–6). Input (4% of total) was also analyzed (lanes 1–3).