Figure 8.

Silencing of RINT-1 causes redistribution of ZW10. (A and B) HeLa cells were transfected without (mock) or with RINT-1 (1149) or lamin A/C siRNA. At 72 h after transfection, the cells were treated with or without 10 μg/ml nocodazole for 90 min. The cells were fixed and double-stained with antibodies against ZW10 and α-tubulin. Bar, 5 μm. (C and D) HeLa cells transfected without (mock) or with ZW10 (102) or lamin A/C siRNA were incubated for 72 h and stained for RINT-1. As observed previously (Hirose et al., 2004), p115 was modestly dispersed in ZW10 (102)-transfected cells (unpublished data), confirming the depletion of ZW10. Alternatively, the cells were incubated with or without 10 μg/ml nocodazole for 90 min, fixed, and double-stained with antibodies against ZW10 and α-tubulin. Expanded images are shown (bottom row). Bar, 5 μm.