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The N terminus of human INCENP (GFP-INCENP1-58) targets to the centromere. HeLa S3 cells were transfected with INCENP1-58 or INCENP59-919 N-terminally fused to GFP. (A) GFP-INCENP1-58 targets to the centromere in prometaphase and metaphase (independently of its expression level) but remains cytoplasmic in anaphase and telophase. (B) A GFP-INCENP construct lacking the first 58 amino acids (GFP-INCENP59-919) does not localize to any defined cellular structure during mitosis. Bar, 10 μm.
