Figure 10.

Model for centromere targeting of the CPC. (A) Our data suggest that Survivin and Borealin, both forming higher molecular order structures at the centromere, bind to the first 58 amino acids of INCENP. Aurora B, in contrast, associates with the C-terminal IN-box of INCENP. We propose that Borealin targets the CPC to the centromere by binding to DNA directly (dashed arrow) but can only do so when present within a functional subcomplex. Aurora B kinase is not involved in this process. (B) The centromere recruitment of the CPC is independent of the CENP-A and the hMis12 assembly pathways. Both pathways are required for the targeting of CENP-I and CENP-H (not depicted); in addition, CENP-A recruits the Hec1/Nuf2-complex. The CPC recruits MCAK to the inner centromere and checkpoint proteins to the kinetochore.