Figure 3.

Genetic analysis of a mutant RSP3 transformant: 388 cells were mated with wt cells and tetrad analysis was performed, revealing a relatively equal occurrence of parental ditype (PD) and nonparental ditype (NPD) tetrads. (A) Western blot analysis of axonemes from a PD tetrad using an antibody to RSP3. Progeny 2B and 2C from the PD tetrad contain the wt form of RSP3 protein, whereas progeny 2A and 2D contain the mutant form of RSP3 protein, as indicated by the migratory shift of the protein band. Axonemal RSP3 from 388 cells and wt cells is shown for comparison. (B) Phenotypic analysis of the tetrad progeny. Live cell motility was assessed for each of the tetrad progeny, showing that the reduced motility phenotype exclusively cosegregates with the mutant form of axonemal RSP3 (2A and 2D).