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. 2001 Jan 23;98(3):1306–1311. doi: 10.1073/pnas.031502498

Figure 4.

Figure 4

Identification of an insertion in MdPI of Rae Ime. (A) Genomic DNA fragments were amplified from Spencer Seedless (1), Wellington Bloomless (2), Rae Ime (3), Granny Smith (4), and Royal Gala (5) by using primers P3 and P7. No DNA was amplified from the control reaction without apple DNA as template (6). (B) Arrows indicate insertion sites in MdPI for Rae Ime (RI), Spencer Seedless (SS), and Wellington Bloomless (WB). The numbers indicate nucleotide positions starting from the ATG codon in the wild-type MdPI of Granny Smith. Dark boxes are coding regions, and the white box is the 3′ noncoding region. Lines between the boxes represent introns. (C) Five-base pair duplication (underlined) of MdPI sequence was found in the insertion site for Rae Ime.