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GST–Chk1 overproduction arrests division in the absence of Wee1 and Cdc25. A wee1-50 Δcdc25 nmt1:GST-chk1+ (BF2404) strain was incubated on medium that repressed (off) or induced (on) GST–Chk1 production. Cells were photographed after 48 h at 35°C.
