Figure 3.

Deletion of mik1⁺ impairs the DNA damage checkpoint. (A) Synchronous cultures of early G₂ wild-type (PR109) or Δmik1 (PR712) cells were produced by centrifugal elutriation and exposed to the radiomimetic drug bleomycin (BL) at a concentration of 2.5 mU/ml or mock treated. (B) Synchronous cultures of early G₂ cdc2-3w Δcdc25 Δmik1 (NR1977) cells were exposed to 200 Gy of ionizing irradiation (+IR) or mock treated (−IR). Note that NR1977 grows slowly relative to cdc2-3w Δcdc25 (GL192) shown in Figure 1A; thus, these experiments cannot be compared directly.