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DNA replication and damage checkpoints stimulate nuclear accumulation of Mik1. Asynchronous populations of strains that contained the mik1+:13Myc allele were incubated in 10 mM hydroxyurea (+HU) or 2.5 mU/ml bleomycin (+BL) for 4 h. Cells were processed to reveal Mik1:13Myc or DNA (DAPI). (A) Wild type (AL2405); (B) Δrad3 (BF2406); (C) Δcds1 (BF2408); (D) Δchk1 (BF2407). (E) Immunoblot analysis of wild-type (AL2405) cells exposed to HU or BL as described above.
