Figure 6.

Chk1-dependent nuclear accumulation of Mik1 is not a consequence of the G₂ arrest. Cultures of cdc25-22 mik1⁺:13Myc (BF2409), cdc25-22 rad3::ura4⁺ mik1:13Myc (BF2442), or cdc25-22 mik1⁺:13Myc Δchk1 (BF2410) cells were shifted to restrictive temperature (36°C). Thirty minutes later, cells were treated with 2.5 mU/ml bleomycin (+BL) or mock treated (−BL). After another 3.5-h incubation at 36°C, cells were processed to detect Mik1:13Myc or DNA (DAPI).