Figure 8.

Accumulation of ubiquitinated Mik1 in proteasome mutant. (A) Wild-type (AL2405) or mts3-1 (NB2411) cells that expressed Mik1:13Myc from the mik1⁺ locus were incubated for 4 h at 35.5°C. Anti-myc immunoblot analysis was performed with cell extracts. (B) The same strains were transformed with pREP1-His₆-ubiquitin and induced to express His₆-ubiquitin before incubation at 4 h at 35.5°C. Samples were processed to purify proteins that were covalently linked to His₆-ubiquitin followed by immunoblot analysis with anti-Myc antibody.