Figure 3.

VSV G proteins slow to exit the ER ultimately reach cell surface. To detect protein at the cell surface, nonpermeabilized BHK-21 cells expressing wild-type or mutant VSV G proteins were fixed and stained with a monoclonal VSV G antibody and a Texas Red-conjugated secondary antibody. Cells were subsequently permeabilized, and intracellular VSV G was detected with an affinity-purified polyclonal anti-VSV G antibody followed by fluorescein-conjugated secondary antibody. Note the prominent Golgi staining present in the cells expressing VSV G and 21_A23_A that is absent in the cells expressing the 19–24_A and CT1 proteins. Bar, 10 μM.