Figure 6.

Cdc42[V36M]p can bind Cdc24p in vitro. (A) In vitro–translated [³⁵S]Cdc24p was incubated with resin-immobilized MBP, MBPCdc42, or MBPCdc42[V36M]. Where indicated, MBP fusions were loaded with GTPγS before binding. Binding reactions were analyzed by SDS-PAGE followed by autoradiography. Standard indicates the percentage of [³⁵S]Cdc24p used in the binding reaction. The two different panels are from two independent experiments. (B) Quantitation of Cdc42p–Cdc24p in vitro bindings. A phosphoimager was used for quantitation. Values are the mean of four independent binding experiments. Error bars, SD. Binding of [³⁵S]Cdc24p to Cdc42p•GTPγS (6.8% of input [³⁵S]Cdc24p) was set to 100%.