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. 2006 Jun;17(6):2824–2838. doi: 10.1091/mbc.E05-11-1040

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© 2006 by The American Society for Cell Biology

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Figure 8. — Overexpression of a fast-cycling Cdc42p mutant partially suppresses the cdc24-m6 cell fusion defect. (A) Expression levels of different Cdc42p versions in CDC24 and cdc24-m6 cells. Extracts of logarithmically growing cells (RAY1042, and RAY1685) carrying pYes plasmid with indicated Cdc42p grown in galactose/fructose containing media were analyzed by immunoblot and probed with anti-HA mAb or anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mAb. Similar results were observed in three independent experiments. (B) Overexpression of fast-cycling Cdc42p partially suppresses the cdc24-m6 cell fusion defect. Cells were grown as described above, mated on galactose/fructose containing media with RAY1487 (GFP-Bud1), and analyzed as in 3D. Values are the means of two independent matings (n = 400 mating pairs). Error bars, SD. (C) Morphology of CDC24 and cdc24-m6 budding cells is unaffected by Cdc42p or Cdc42[C18A]p overexpression. DIC Images of indicated cells used for above matings grown in galactose/fructose containing media.