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. 2006 Jun;17(6):2824–2838. doi: 10.1091/mbc.E05-11-1040

Table 1.

Yeast strains used in this study

Strain Genotypea Source
JY426 MATa, leu2-3,-112, ura3-52, his4-34, fus1-Δ1, fus2-Δ3 Cold Spring Harbor Laboratory
JY429 MATα, trp1Δ1, ura3-52, cyh2, fus1-Δ1, fus2-Δ3 Cold Spring Harbor Laboratory
SEY6210 MATα, leu2-3,-112, ura3-52, his3-Δ200, trp1-Δ901, lys2-801, suc2-Δ9 S. Emr
SEY6211 MATa, leu2-3,-112, ura3-52, his3-Δ200, trp1-Δ901, ade2, suc2-Δ9 S. Emr
RAY399 MATα/a, leu2-3,-112/leu2-3,-112, ura3-52/ura3-52, his3-Δ200/his3-Δ200, trp1-Δ901/trp1-Δ901, LYS2/lys2-801, ADE2/ade2, suc2-Δ9/suc2-Δ9 This studyb
RAY485 RAY399 CDC42/cdc42-Δ1::LEU2 This study
RAY513 SEY6210 cdc42-Δ1::LEU2 with pRS424GALGFPCDC42 This studyc
RAY563 SEY6210 sph1-Δ1::HIS3 Arkowitz and Lowe (1997)
RAY567 SEY6211 sph1-Δ1::HIS3 Arkowitz and Lowe (1997)
RAY950 MATa, leu2-3,-112, ura3-52, his3-Δ200, trp1-Δ901, lys2-801, ade2, cdc24::LEU2 with pRS416GalHis6CDC24 Nern and Arkowitz (1998)
RAY1042 RAY950 with pRS414CDC24 instead of pRS416GalHis6CDC24 Nern and Arkowitz (1998)
RAY1052 SEY6211 cdc24-Δ1::LoxP with pEG(KT)CDC24 Nern and Arkowitz (2000a)
RAY1142 SEY6211 cdc24::TRP1 cdc24-m1, bud1Δ::LoxP HIS5Sp LoxP Nern and Arkowitz (1999)
RAY1487 SEY6211 cdc24::TRP1 CDC24, URA3::GFPBUD1 Nern and Arkowitz (2000a)
RAY1556 SEY6210 cdc42-Δ1::LEU2 with pRS416CDC42 This studyd
RAY1565 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36M, P69T] This studyd,e
RAY1635 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36M] This studyd,f
RAY1638 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[F37I] This studyd,f
RAY1640 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36A, A41G] This studyd,f
RAY1685 RAY950 with pRS414cdc24-m6 instead of pRS416GalHis6CDC24 Barale et al. (2004)
RAY1728 SEY6211 cdc24-Δ1::LoxP, cdc42-Δ1::LEU2 with pRS414CDC24 and pRS416CDC42 This studyg
RAY1772 SEY6211 cdc42-Δ2::HIS5Sp with pRS416CDC42 This study
RAY1793 SEY6211 cdc24-Δ1::LoxP, cdc42-Δ1::LEU2 with pRS414CDC24 and pRS416cdc42[V36V]i This studyh
RAY1801 SEY6211 cdc24-Δ1::LoxP, cdc42-Δ1::LEU2 with pRS414cdc24-m6 and pRS416cdc42[V36V]i This studyj
RAY1830 SEY6211 cdc24-Δ1::LoxP, cdc42-Δ1::LEU2 with pRS414CDC24 and pRS413GFPCDC42 This studyh
RAY1833 SEY6211 cdc24-Δ1::LoxP, cdc42-Δ1::LEU2 with pRS414cdc24-m6 and pRS413GFPCDC42 This study
RAY1907 SEY6210 sph1-Δ1::HIS3, URA3::GFPBUD1 This study
RAY1912 SEY6210 cdc42-Δ1::LEU2 with pRS414cdc42[V36V]i This study
RAY1914 SEY6210 cdc42-Δ1::LEU2 with pRS414cdc42[V36M] This study
RAY1918 SEY6211 cdc42-Δ2::HIS5Sp with pRS414cdc42[V36V]i This study
RAY1920 SEY6211 cdc42-Δ2::HIS5Sp with pRS414cdc42[V36M] This study
RAY1926 SEY6211 cdc42-Δ2::HIS5Sp with pRS416cdc42[V36M] This study
RAY1951 SEY6211 cdc24-Δ1::LoxP, cdc42-Δ1::LEU2 with pRS414CDC24 and pRS413GFPcdc42[V36M] This study
RAY1952 SEY6211 cdc24-Δ1::LoxP, cdc42-Δ1::LEU2 with pRS414CDC24 and pRS416cdc42[V36M] This studyk
DMY13 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36W] This studyd
DMY14 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36C] This studyd
DMY15 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36F] This studyd
DMY16 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36L] This studyd
DMY17 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36I] This studyd
DMY18 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36A] This studyd
DMY19 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36T] This studyd
DMY20 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36Y] This studyd
DMY21 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36Q] This studyd
DMY22 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36S] This studyd
DMY23 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36H] This studyd
DMY25 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36K] This studyd
DMY26 SEY6210 cdc42-Δ1::LEU2 with pRS416cdc42[V36R] This studyd

aHIS5Sp refers to HIS5 from Schizosaccharomyces pombe.

b Constructed by mating SEY6210 and SEY6211.

c Plasmid pRS424GALGFPCDC42 was transformed into RAY399 and tetrads were dissected.

d Indicated plasmid pRS416CDC42 or cdc42 mutant was transformed into RAY513 and pRS424GALGFPCDC42 was cured.

e Isolated in first cdc42 mutant screen.

f Isolated in second cdc42 mutant screen.

g RAY1052 was transformed with pRS414CDC24 and plasmid pEG(KT)CDC24 was cured. This strain was then crossed to RAY1556 followed by sporulation.

h Constructed by transformation of RAY1728 with pRS413GFPCDC42 and pRS416CDC42 was cured (RAY1830). Subsequently strain was transformed with indicated pRS416cdc42 plasmid and plasmid pRS413GFPCDC42 was cured.

i Cdc42[V36V] was generated by site-directed mutagenesis of Cdc42p replacing Val 36 with Val (in addition to a silent SnaBI site).

j Constructed by transformation of RAY1793 with pRS313CDC24 and pRS414CDC24 was cured. Subsequently strain was transformed with pRS414cdc24-m6 plasmid and plasmid pRS313CDC24 was cured.

k Constructed by transformation of RAY1793 with pRS413GFPCDC42 and pRS416cdc42[V36V] was cured. Subsequently the strain was transformed with pRS416cdc42[V36M] plasmid and plasmid pRS413GFPCDC42 was cured.