Table 3.
Morphology, budding pattern, and mating efficiency of cdc42 mutants
| Amino acid 36 | Viability | Morphologya | Axial bud site selectionb | Mating efficiencyc |
|---|---|---|---|---|
| Val | + | I | 89 | ++ |
| Ala | + | II | 65 | + |
| Leu | + | I | nd | ++ |
| Ile | + | I | nd | ++ |
| Met | + | I/II | 75 | − |
| Phe | + | II | nd | nd |
| Ser | + | I | 70 | ++ |
| Thr | + | II/III | nd | + |
| Tyr | + | III | nd | ++ |
| Trp | + | III | 49 | − |
| Gln | + | I | nd | + |
| Cys | + | I | 89 | ++ |
| Lys | + | II | 58 | + |
| Arg | + | II | 70 | nd |
| His | + | II/III | 22 | + |
| Glu | − | nd | nd | nd |
a Wild-type morphology (I), elongated cells (II), and large cells (III).
b Percentage axial budding pattern (n = 100).
c Plate mating efficiency with an enfeebled tester; ++, wild-type efficiency; +, slightly defective; and −, strongly defective.