Figure 3.

Behavior of GFP-Snc1p chimeras with different transmembrane domains. The chimera TMD sequences and their locations are listed in Table 2; examples are shown here. Snc refers to GFP-Snc1p. Hyphenated names indicate chimeras with TMDs derived from Sso1p (sso), Nyv1p (nyv), Ufe1p with insertions to make the TMD 18 and 26 residues long (ufe18 and ufe26), or the sequence called random1 in Table 2 (rnd1). These were expressed in wild-type (wt), end4, or tlg mutants as indicated. Constructs labeled en⁻ had in addition the point mutations that inhibit endocytosis (V40A and M43A). The wild-type cells expressing Snc-nyv were labeled with FM4-64 to reveal vacuoles, and both images are shown.