Immunofluorescence of late Golgi/endosomal markers. Pairs of panels show fixed cells double labeled with appropriate antibodies or, in the case of Sec7p and the L96V mutant of GFP-Snc1p, with GFP. Arrows indicate equivalent positions. Note extensive colocalization of Tlg1p, Tlg2p, Kex2p, and the L96V mutant but limited overlap between Tlg1p or Tlg2p and Sec7p.