Table 2.
Fate of GFP-Snc1p constructs with altered transmembrane domains
| TMD sequence | Length | Origin | Location in end4 | Location (en−) | Location in wild type |
|---|---|---|---|---|---|
| KMCLALVIIILLVVIIVPIAVHFSR* | 20 | Snc1p | PM | PM | PM |
| KCWLIVFAIIVVVVVVVVVPAVVKTR* | 22 | Sso1p | PM | PM | Vac |
| KNITLLTFTIILFVSAAFMFFYLW* | 23 | Nyv1p | Vac | Vac | Vac |
| KLTTYGAIIMGVFILFLVLDYVG* | 18 | “Ufe1p” | Vac | Vac | Vac |
| KLTTYGAIIMGVFILFLVLVLDYVG* | 20 | “Ufe1p” | Vac | Vac | |
| KLTTYGAIIMGVFILFLVLVLVLDYVG* | 22 | “Ufe1p” | Vac | Vac | |
| KLTTYGAIIMGVFILFLVLVLVLVLVLDYVG* | 26 | “Ufe1p” | PM | PM | Vac |
| KVVVVVVVVVVVVVVVVVVVVVVVVVVHFSR* | 26 | Synthetic | PM | Vac | |
| KVFVIIFLVLLMVILLLVVLVHFSR* | 20 | Random 1 | PM | Vac | |
| KIVVVLILIFVIIIIMLLLILHFSR* | 20 | Random 2 | PM | Vac | |
| KLVLLLLFLLMFLLILLLVILHFSR* | 20 | Random 3 | PM | Vac | |
| KVLILIFVLFIIFILVIVLVIIL* | 22 | Random 4 | PM | Vac |
The TMD and following residues were exchanged for those shown. “Ufe1p” refers to lengthened versions of the TMD of Ufe1p, inserted residues being underlined in the sequence. PM, plasma membrane; Vac, vacuole.