Table II.

Amount of PvPGIP1 bound to polyacrylamide gel columns containing different cell wall fractions

The Ca²⁺-wall fraction/polyacrylamide columns equilibrated with 20 mm sodium acetate buffer, pH 5.0, containing 100 μm CaCl₂ were loaded with PvPGIP1, extensively washed with the equilibration buffer, and eluted with PBS containing 0.3 m NaCl. The control was performed by loading PvPGIP1 onto a polyacrylamide gel column; n.d. stands for not detectable. The percentage of bound PGIP with respect to the amount of loaded PGIP (100%) was determined by inhibition assays against AnPGII. The percentage reported is the average (±sd) of three different experiments.

Column Containing:	Bound PGIP
	%
AIS	58 (±4)
BSS	38 (±3)
ChASS	63 (±6)
Residue	7 (±2)
Control	n.d.