Figure 3.

Web user interfaces. FTFLPdb and associated Web applications have several functionalities including Search, Submit, Edit, Assign, Submit/Edit, Get Primers, Download Clone Submission Data, and Download Seed Submission Data. A, A search page provides two categories of parameters, gene related and protein related, to search for genes of interest. B, Search result page shows general annotation of queried genes, which includes gene length, protein M_r, protein length, GFP insertion position, whether it is a membrane protein, gene expression level, EST number, taxon group, gene alias, and membership in a gene family. Camera or book icon indicates that the gene product has been localized in the cell empirically. C, Detailed information of one of the subcellular localization images of protein AT2G04410.1 is shown. D, The subcellular localization image of protein AT2G04410.1, which localizes to the cell wall and extracellular matrix, is shown. AT2G00410-GFP was expressed under a native promoter. The green shows expression of the protein in the secondary cell wall of endothelium cells and in the extracellular matrix of mature pollens. Autofluorescence in chlorophyll is shown in red. E, Primer information page provides primer sequence, length, and Tm. P1 and P4 primers contain sequences 5′-gctcgatccacctaggct-3′ and 5′-cgtagcgagaccacagga-3′, respectively, that partially overlap the Gateway primers, whereas P2 and P3 primers contained sequences 5′-cacagctccacctccacctccaggccggcc-3′ and 5′-tgctggtgctgctgcggccgctggggcc-3′, respectively, that partially overlap the GFP tag linkers. The capital letters are primer sequences matching the target gene sequence. F, Primer map shows the positions of primers on the target gene (red, coding region; blue, noncoding region). The two numbers within the parentheses show the start position (first no.) and the primer length (second no.). The italic letters show primer sequence. The underline indicates the primer position.