Figure 4.

Interaction of ABI3 and WRI1. A, Germination assays on early desiccation seeds of wild type (black bars), abi3-3 (densely hatched bars), abi3-3 wri1-1 (sparsely hatched bars), and wri1-1 (white bars) are shown. ABA was present (+) at 10 μm or absent (−). Absolute percent germination is reported as the average of the mean of three independent experiments with the error bars representing sd. B, mRNA abundance of WRI1 and ABI3 in dry seeds (D), 16 h (16), and 48 h (48) after imbibition. The RNA was blotted and the blots probed with the indicated gene. The rRNA bands from the ethidium bromide-stained gel are shown as a loading control.