Figure 5.

The N-terminal region of CmERS1 is located in the lumen. A, Schematic of TM (M2N)-GFP construct. An N-glycosylation site was introduced to the N terminus of the TM1-2-3-GFP fusion by substituting Asn for Met-2. B, The plasmid was transcribed and translated in vitro in the absence (−) or presence (+) of RM. Endo H treatment was performed at 37°C for 6 h. PK digestion and alkali extraction were performed as described in Figure 4. The glycosylated form is indicated by an asterisk.