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. 2006 Jun;18(6):1396–1411. doi: 10.1105/tpc.105.039008

Figure 3.

Figure 3.

Real-Time RT-PCR Analysis of AGD1, AGD2, AGD4, and SFC Expression.

(A) Relative expression of class 1 ARF-GAP domain genes in RNA isolated from roots, hypocotyl, cotyledons, leaves plus meristem of 8-d-old (22°C-grown) seedlings, 12-d-old whole seedlings, and mixed-age siliques of mature Landsberg erecta (Ler) plants. Expression was normalized to β-tubulin and shown relative to the expression of SFC in RNA isolated from leaf and shoot apical meristem combined.

(B) Relative transcript levels in agd2 and agd4 mutants. RNA was isolated from 8-d-old roots (of the wild type and agd2 mutants) and 8-d-old cotyledons (of the wild type and agd4 mutants). Transcript levels were measured using real-time RT-PCR, normalized to β-tubulin, and are shown relative to expression of that gene (AGD2 or AGD4) in the wild-type tissue.

Light-gray bars represent SFC, dark-gray bars represent AGD1, white bars represent AGD2, and black bars represent AGD4. θ indicates no expression detected. Error bars represent sd (two to six experiments).