Figure 8.

sfc Roots Transport and Respond to Exogenous Auxin.

(A) Schematic diagram of auxin transport assays. The gray bar represents an agar block containing [³H]-IAA, which was placed at the root-shoot junction. The dashed line indicates that portion of the root that was discarded (2 mm). Lines between the two plants indicate the portions of the roots where [³H]-IAA was measured.

(B) Bar graph depicting acropetally transported [³H]-IAA. Tissue measured is diagramed in (A). The set to the left shows measurements of two root segments, while the center set depicts an independent measurement of three root segments. The measurements to the right show the influence of 10 μM NPA on acropetally transported [³H]-IAA in the upper segment of the wild type and sfc mutants. Similar results were obtained in the other segments. Bars indicate averages ± sd.

(C) Lateral root numbers of sfc and wild-type decapitated roots. The controls are nondecapitated (intact) plants; bars to the right are numbers for plants where an agar block containing no [³H]-IAA or 10 μM was applied at the cut site (root-shoot junction). Lateral root counts were made 3 d after auxin application. Bars indicate averages ± sd.