Figure 9.

Confocal Analysis of PIN1:GFP and Controls Reveals Cycling Defects in sfc Mutants.

The wild type and sfc mutants show similar patterns of V6 and Q4 controls (streaming dots and endoplasmic reticulum surface) and similar patterns of PIN1:GFP except following treatment with BFA. In sfc mutants, BFA treatment results in PIN1:GFP accumulation in many small compartments in contrast with the two to three large compartments in the wild type. Bars = 10 μm, except for the two top left low-magnification images of PIN1:GFP, in which bars = 100 mm.