Figure 2.

Alb3.1 and Alb3.2 Are Part of High Molecular Mass Complexes.

(A) Thylakoid membranes from wild-type cells. The membranes were solubilized with β-dodecyl maltoside and fractionated by centrifugation on a linear 0.1 to 2 M sucrose gradient for 16 h at 170,000g. Fractions were used for immunoblotting with the indicated antibodies.

(B) Thylakoid membranes from the ac29 strain containing HA-tagged Alb3.1 and FLAG-tagged Alb3.2. Membranes were solubilized and fractionated as described for (A). Fractions were used for immunoblotting with the HA, FLAG, D2, and PsaA antibodies.

The lower Alb3.2 band is unspecific, as it is also observed with total protein extracts from an untagged wild-type strain (data not shown). Size markers are indicated.