Figure 4.

Comigration of Alb3.2 with PSII Complexes on BN Gels.

(A) Total membranes from the wild type and the mutant strains ac29, FuD7, F15, ΔpetD, and FuD50. The membranes were solubilized with β-dodecyl maltoside and fractionated by BN gel electrophoresis. The antibodies used for immunoblots are indicated at top. The arrow indicates the position of the presumed free Alb3.2.

(B) Analysis of Alb3.2 in y-1. The y-1 mutant was grown in darkness and shifted to the light at 0 h. Total membranes were prepared at different times of the greening process and fractionated by BN gel electrophoresis (Gel). Immunoblotting was performed with Alb3.2 antiserum (Alb3.2). The location of the PSII core complex is indicated. CL, growth in continuous light.

(C) Accumulation of proteins during greening of y-1. Total proteins from y-1 and the wild type were extracted during the greening process and fractionated by PAGE. Immunoblotting was performed with antisera raised against the indicated proteins. Rubisco, ribulose-1,5-bis-phosphate carboxylase/oxygenase.